Ask Mr. Wizard Yeast Propagation From a Bottle Q If I am starting from commercial bottle dregs for yeast harvesting, Can I just pitch the dregs into a 3-L (3-qt.) starter at 1.035–1.040 that is well fed, oxygenated, and on a stir plate; or do I need to step through multiple stages to get to my target volume, like most of the literature out there tells me to? Why can't I just target making a single, end-point volume starter, and expect ~150 billion cells per liter as an outcome? A I love these two part questions that begin with great fundamentals and then segue into the meaning of life. You want to propagate yeast from bottles and are attracted to skipping the intermediate steps mentioned in just about every brewing text that covers yeast propagation. For the benefit of readers who are not up on these methods, I will briefly cover the basics of how a brewer with a basic home microbiology lab can propagate yeast from a bottle of beer. We need an example for this question and I am going to take the easy route and select a bottle of Sierra Nevada Pale Ale. Read more |
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